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 Anti-Bovine Factor V (5105)

PRODUCT AND PURCHASING INFORMATION

 

Description:

Anti-Bovine Factor V antibody

Catalog Number:

ABV-5105

Type/Host:

Monoclonal; mouse

Subclass:

IgG1

Clone Number:

ABV-5105

Immunogen:

Purified Bovine factor V

Purity:

>95% by SDS-PAGE

Epitope:

N-terminal of the 74,000 Da light chain

Dissociation Constant:

Not determined

Inhibitory Properties:

Not inhibitory

Specificity:

Bovine factor Va light chain, bovine factor V in the absence of calcium.  Does not react with murine factor V (Western blot on mouse plasma)

Size:

100 µg; Bulk quantities are available.

Formulation:

50% Glycerol/H2O (vol/vol) 

Storage:

-20oC

Shelf Life (properly stored):

12 months
 
Applications: - Radioimmunoassay (RIA) - Yes
- Blot - Yes (suggested starting concentration of 5 µg/mL)
- ELISA - Not determined
- Useful for purification - No
General Application Guidelines: Western blot, ELISA, Immunohistochemistry
Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded (ex. Immuno Histochemistry).
 

Customization options:

- Reformulation into the buffer of your choice
- Custom aliquot sizes
- Conjugation with FITC, Biotin, HRP and other conjugates
- Coupling to sepharose and column preparation
 

Sample Gel:

 

Gel: Novex 4-12% Bis-Tris

Load: 1 µg per lane

Buffer: MOPS

Standard: SeeBluePlus 2; Myosin (191 kDa), Phosphorylase B (97 kDa), BSA (64 kDa), Glutamic Dehydrogenase (51 kDa), Alcohol Dehydrogenase (39 kDa), Carbonic Anhydrase (28 kDa), Myoglobin Red (19 kDa), Lysozyme (14 kDa)

 

 

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