|
Description: |
Anti-Bovine
Factor V antibody |
|
Catalog
Number: |
ABV-5106 |
|
Type/Host: |
Monoclonal;
mouse |
|
Subclass: |
IgG1 |
|
Clone
Number: |
ABV-5106 |
|
Immunogen: |
Purified
Bovine factor V |
|
Purity: |
>95%
by SDS-PAGE |
|
Epitope: |
N-terminal
of the 94,000 Da heavy chain |
|
Dissociation
Constant: |
Not
determined |
|
Inhibitory
Properties: |
Not
inhibitory |
|
Specificity: |
Bovine
factor Va heavy chain, weakly with intact bovine factor V. Does
not recognize murine factor V via Western blot on plasma samples. |
|
Size: |
100
µg; Bulk quantities are available. |
|
Formulation: |
50%
Glycerol/H2O (vol/vol) |
|
Storage: |
-20oC |
|
Shelf
Life (properly stored): |
12
months |
| |
| Applications: |
- Radioimmunoassay
(RIA) - Not determined |
|
- Blot
- Yes (suggested starting concentration of 5 µg/mL) |
|
- ELISA
- Yes |
|
- Useful
for purification - No |
| General
Application Guidelines: Western
blot, ELISA,
Immunohistochemistry |
| Each
laboratory should determine an optimum working titer for use in its
particular application. Other applications have not been tested but
use in such assays should not necessarily be excluded (ex. Immuno
Histochemistry). |
| |
|
Customization
options: |
- Reformulation
into the buffer of your choice |
|
- Custom
aliquot sizes |
|
- Conjugation
with FITC, Biotin, HRP and other conjugates |
|
- Coupling
to sepharose and column preparation |
| |
|
Sample Gel:
|
Gel:
Novex 4-12% Bis-Tris
Load: 1 µg per lane
Buffer:
MOPS
Standard:
SeeBluePlus 2; Myosin (191 kDa), Phosphorylase B (97 kDa), BSA (64
kDa), Glutamic Dehydrogenase (51 kDa), Alcohol Dehydrogenase (39 kDa),
Carbonic Anhydrase (28 kDa), Myoglobin Red (19 kDa), Lysozyme (14
kDa)
|
| |
|
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