|
Description: |
Anti-Bovine
Factor X antibody |
|
Catalog
Number: |
ABX-5051 |
|
Type/Host: |
Monoclonal;
mouse |
|
Subclass: |
IgG1 |
|
Clone
Number: |
ABX-5051 |
|
Immunogen: |
Purified
bovine factor X |
|
Purity: |
>95%
by SDS-PAGE |
|
Epitope: |
Not
determined |
|
Dissociation
Constant: |
Kd
= 9X10-11M |
|
Inhibitory
Properties: |
Inhibitory
in clotting assay (APTT and PT), partial calcium dependence |
|
Specificity: |
Heavy
chain of factor X and Xa (reactive toward human, bovine, rabbit, sheep,
porcine and canine factor X), BEGRck factor Xa. Does not recognize murine
X (via Western blot). |
|
Size: |
100
µg; Bulk quantities are available. |
|
Formulation: |
50%
Glycerol/H2O (vol/vol) |
|
Storage: |
-20oC |
|
Shelf
Life (properly stored): |
12
months |
| |
| Applications: |
- Radioimmunoassay
(RIA) - Yes |
|
- Blot
- Yes (suggested starting concentration of 5 µg/mL) |
|
- ELISA
- Unknown |
|
- Useful
for purification - Yes |
| General
Application Guidelines: Western
blot, ELISA,
Immunohistochemistry |
| Each
laboratory should determine an optimum working titer for use in its
particular application. Other applications have not been tested but
use in such assays should not necessarily be excluded (ex. Immuno
Histochemistry). |
| |
|
Customization
options: |
- Reformulation
into the buffer of your choice |
|
- Custom
aliquot sizes |
|
- Conjugation
with FITC, Biotin, HRP and other conjugates |
|
- Coupling
to sepharose and column preparation |
| |
|
Sample Gel:
|
 Gel:
Novex 4-12% Bis-Tris
Load: 1 µg per lane
Buffer:
MOPS
Standard:
SeeBluePlus 2; Myosin (191 kDa), Phosphorylase B (97 kDa), BSA (64
kDa), Glutamic Dehydrogenase (51 kDa), Alcohol Dehydrogenase (39 kDa),
Carbonic Anhydrase (28 kDa), Myoglobin Red (19 kDa), Lysozyme (14
kDa)
|
| |
|
U.S.
PRICING PRODUCT
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DATA SHEET ORDER
NOW! |
