|
Description: |
Anti-Human
Factor IX antibody |
|
Catalog
Number: |
AHIX-5041 |
|
Type/Host: |
Monoclonal;
mouse |
|
Subclass: |
IgG1 |
|
Clone
Number: |
AHIX-5041 |
|
Immunogen: |
Purified
human factor IX |
|
Purity: |
>95%
by SDS-PAGE |
|
Epitope: |
Not
determined |
|
Dissociation
Constant: |
Not
determined |
|
Inhibitory
Properties: |
Not
inhibitory |
|
Specificity: |
Human
factor IX, human factor IXa, heavy chain of human factor IX and IXa.
Does not recognize murine factor IX via Western blot on plasma. |
|
Size: |
100
µg; Bulk quantities are available. |
|
Formulation: |
50%
Glycerol/H2O (vol/vol) |
|
Storage: |
-20oC |
|
Shelf
Life (properly stored): |
12
months |
| |
| Applications: |
- Radioimmunoassay
(RIA) - Yes |
|
- Blot
- Yes (suggested starting concentration of 5 µg/mL) |
|
- ELISA
- Yes |
|
-
Immunohistochemistry - Yes |
|
-
Useful for purification - Yes |
| General
Application Guidelines: Western
blot, ELISA,
Immunohistochemistry |
| Each
laboratory should determine an optimum working titer for use in its
particular application. Other applications have not been tested but
use in such assays should not necessarily be excluded. |
| |
|
Customization
options: |
- Reformulation
into the buffer of your choice |
|
- Custom
aliquot sizes |
|
- Conjugation
with FITC, Biotin, HRP and other conjugates |
|
- Coupling
to sepharose and column preparation |
| |
|
Sample Gel:
|
 Gel:
Novex 4-12% Bis-Tris
Load: 1 µg per lane
Buffer:
MOPS
Standard:
SeeBluePlus 2; Myosin (191 kDa), Phosphorylase B (97 kDa), BSA (64
kDa), Glutamic Dehydrogenase (51 kDa), Alcohol Dehydrogenase (39 kDa),
Carbonic Anhydrase (28 kDa), Myoglobin Red (19 kDa), Lysozyme (14
kDa)
|
| |
|
Sample
publications using this antibody:
-
As
capture in ELISA (Jiang, H., et al., Blood 2006 Nov 15; 108(10):
3321-3328 doi:10.1182/blood-2006-04-017913)
-
As
capture in ELISA (Rehemtulla, A,. et al,. Proc.
Natl. Acad. Sci. USA (1993) 90: 4611-4615)
This
publication list is not all encompassing, and is only meant to
provide limited examples of how Haematologic Technologies' products
are used. We encourage you to search the literature for other
examples pertinent to your experimentation, and to contact us with
any technical questions. |
|
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