|
Description: |
Sheep
Anti-Human Factor VIII antibody |
|
Catalog
Number: |
PAHFVIII-S |
|
Type/Host: |
Polyclonal;
Sheep. Source: sheep serum |
|
Description: |
Purified
IgG fraction |
|
Immunogen: |
Human
Factor VIII:C (Factor VIII purified from factor VIII concentrate) |
|
Purity: |
>95%
by SDS-PAGE |
|
Inhibitory
Properties: |
Inhibitor
activity of this IgG was measured by Bethesda-style inhibitor assay
where it had an activity of 77 B.U./mg IgG. |
|
Specificity: |
Human
Factor VIII; Immunodiffusion (+), ELISA (+). No reactivity was observed
with von Willebrand Factor
|
|
Size: |
1
and 5 mg; Bulk quantities are available. |
|
Formulation: |
50%
Glycerol/H2O (vol/vol) |
|
Storage: |
-20oC |
|
Shelf
Life (properly stored): |
12
months |
| |
| Applications: |
-
useful in Immunoassays of Factor VIII concentrate, Factor VIII
concentrate-vWF concentrate |
|
-
useful in neutralization studies where antibodies are required to
inhibit the activity of Factor VIII |
|
|
| General
Application Guidelines: Western
blot, ELISA,
Immunohistochemistry |
| Each
laboratory should determine an optimum working titer for use in its
particular application. Other applications have not been tested but
use in such assays should not necessarily be excluded (ex. Immuno
Histochemistry). |
| |
|
Customization
options: |
- Reformulation
into the buffer of your choice |
|
- Custom
aliquot sizes |
|
- Conjugation
with FITC, Biotin, HRP and other conjugates |
|
- Coupling
to sepharose and column preparation |
| |
|
Sample Gel:
|
 Gel:
Novex 4-12% Bis-Tris
Load: 1 µg per lane
Buffer:
MOPS
Standard:
SeeBluePlus 2; Myosin (191 kDa), Phosphorylase B (97 kDa), BSA (64
kDa), Glutamic Dehydrogenase (51 kDa), Alcohol Dehydrogenase (39 kDa),
Carbonic Anhydrase (28 kDa), Myoglobin Red (19 kDa), Lysozyme (14
kDa)
|
| |
|
Sample
publications using this antibody:
-
Blot of
factor VIII light chain (Chen, L., et al., Mol Ther.
2007 October ; 15(10): 1856–1862. doi:10.1038/sj.mt.6300268)
This
publication list is not all encompassing, and is only meant to provide
limited examples of how Haematologic Technologies' products are used.
We encourage you to search the literature for other examples pertinent
to your experimentation, and to contact us with any technical questions. |
|
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