|
Description: |
Anti-Human
Factor V antibody |
|
Catalog
Number: |
AHV-5108 |
|
Type/Host: |
Monoclonal;
mouse |
|
Subclass: |
IgG |
|
Clone
Number: |
AHV-5108 |
|
Immunogen: |
Purified
human factor V |
|
Purity: |
>95%
by SDS-PAGE |
|
Epitope: |
C-terminus
of the 74kDa light chain of factor V |
|
Dissociation
Constant: |
Not
determined |
|
Inhibitory
Properties: |
Not
inhibitory |
|
Specificity: |
Human
factor V and Va, light chain (fragment E, 74,000) of factor Va.
Does not recognize murine factor V via Western blot. |
|
Size: |
100
µg; Bulk quantities are available. |
|
Formulation: |
50%
Glycerol/H2O (vol/vol) |
|
Storage: |
-20oC |
|
Shelf
Life (properly stored): |
12
months |
| |
| Applications: |
- Radioimmunoassay
(RIA) - Yes |
|
- Blot
- Yes (suggested starting dilution is 5 µg/mL) |
|
- Useful
for purification - No |
|
- ELISA
- not known |
| General
Application Guidelines: Western
blot, ELISA,
Immunohistochemistry |
| Each
laboratory should determine an optimum working titer for use in its
particular application. Other applications have not been tested but
use in such assays should not necessarily be excluded (ex. Immuno
Histochemistry). |
| |
|
Customization
options: |
- Reformulation
into the buffer of your choice |
|
- Custom
aliquot sizes |
|
- Conjugation
with FITC, Biotin, HRP and other conjugates |
|
- Coupling
to sepharose and column preparation |
| |
|
Sample Gel:
|
Gel:
Novex 4-12% Bis-Tris
Load: 1 µg per lane
Buffer:
MOPS
Standard:
SeeBluePlus 2; Myosin (191 kDa), Phosphorylase B (97 kDa), BSA (64
kDa), Glutamic Dehydrogenase (51 kDa), Alcohol Dehydrogenase (39 kDa),
Carbonic Anhydrase (28 kDa), Myoglobin Red (19 kDa), Lysozyme (14
kDa)
|
| |
|
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