|
Description: |
Sheep
Anti-Human Factor V antibody |
|
Catalog
Number: |
PAHFV-S |
|
Type/Host: |
Polyclonal;
Sheep. Source: sheep serum |
|
Description: |
Purified
IgG fraction |
|
Immunogen: |
Purified
human factor V |
|
Purity: |
>95%
by SDS-PAGE |
|
Inhibitory
Properties: |
Not
determined |
|
Specificity: |
Human
factor V |
|
Size: |
1
and 5 mg; Bulk quantities are available. |
|
Formulation: |
50%
Glycerol/H2O (vol/vol) |
|
Storage: |
-20oC |
|
Shelf
Life (properly stored): |
12
months |
| |
| Applications: |
- Radioimmunoassay
(RIA) - Not determined |
|
- Blot
- Yes (suggested starting concentration of 10 µg/mL) |
|
- ELISA
- Yes (suggested starting concentration of 10 µg/mL) |
| General
Application Guidelines: Western
blot, ELISA,
Immunohistochemistry |
| Each
laboratory should determine an optimum working titer for use in its
particular application. Other applications have not been tested but
use in such assays should not necessarily be excluded (ex. Immuno
Histochemistry). |
| |
|
Customization
options: |
- Reformulation
into the buffer of your choice |
|
- Custom
aliquot sizes |
|
-
Affinity purification |
|
- Conjugation
with FITC, Biotin, HRP and other conjugates |
|
- Coupling
to sepharose and column preparation |
| |
|
Sample Gel:
|
 Gel:
Novex 4-12% Bis-Tris
Load: 1 µg per lane
Buffer:
MOPS
Standard:
SeeBluePlus 2; Myosin (191 kDa), Phosphorylase B (97 kDa), BSA (64
kDa), Glutamic Dehydrogenase (51 kDa), Alcohol Dehydrogenase (39 kDa),
Carbonic Anhydrase (28 kDa), Myoglobin Red (19 kDa), Lysozyme (14
kDa)
|
| |
|
Sample
publications using this antibody:
-
Blot of
factor V (Zeibdawi, A. and Pryzdial, E. Biochem. J.
2001 June 8; 276(23): 19929-19936)
-
Blot
of factor V (Zeibdawi, A., et al.,
Biochem. J. (2004) 377, 141–148)
This
publication list is not all encompassing, and is only meant to provide
limited examples of how Haematologic Technologies' products are used.
We encourage you to search the literature for other examples pertinent
to your experimentation, and to contact us with any technical questions. |
|
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