|
Description: |
Anti-Human
Factor X antibody |
|
Catalog
Number: |
AHX-5050 |
|
Type/Host: |
Monoclonal;
mouse |
|
Subclass: |
IgG1 |
|
Clone
Number: |
AHX-5050 |
|
Immunogen: |
Purified
human factor X |
|
Purity: |
>95%
by SDS-PAGE |
|
Epitope: |
Not
determined |
|
Dissociation
Constant: |
Not
determined |
|
Inhibitory
Properties: |
Inhibits
PT, prothrombinase and partially APTT, but not amidase activity. |
|
Specificity: |
Heavy
chains of human factors Xa and X; does not bind bovine factor X or
BEGRck-Xa. Does not recognize murine or porcine X via Western
blot. |
|
Size: |
100
µg; Bulk quantities are available. |
|
Formulation: |
50%
Glycerol/H2O (vol/vol) |
|
Storage: |
-20oC |
|
Shelf
Life (properly stored): |
12
months |
| |
| Applications: |
- Radioimmunoassay
(RIA) - Not determined |
|
- Blot
- Yes (suggested starting concentration of 5 µg/mL) |
|
- ELISA
- Yes |
|
- Useful
for purification - Yes |
| General
Application Guidelines: Western
blot, ELISA,
Immunohistochemistry |
| Each
laboratory should determine an optimum working titer for use in its
particular application. Other applications have not been tested but
use in such assays should not necessarily be excluded (ex. Immuno
Histochemistry). |
| |
|
Customization
options: |
- Reformulation
into the buffer of your choice |
|
- Custom
aliquot sizes |
|
- Conjugation
with FITC, Biotin, HRP and other conjugates |
|
- Coupling
to sepharose and column preparation |
| |
|
Sample Gel:
|
 Gel:
Novex 4-12% Bis-Tris
Load: 1 µg per lane
Buffer:
MOPS
Standard:
SeeBluePlus 2; Myosin (191 kDa), Phosphorylase B (97 kDa), BSA (64
kDa), Glutamic Dehydrogenase (51 kDa), Alcohol Dehydrogenase (39 kDa),
Carbonic Anhydrase (28 kDa), Myoglobin Red (19 kDa), Lysozyme (14
kDa)
|
| |
|
Sample
publications using this antibody:
-
Immunoblot
of FX (Simioni, P., et al., Thrombosis Research 2001 Feb 15;
101(4): 219-230 doi:10.1016/S0049-3848(00)00406-0)
-
Effect
on prothrombin activation (Geaquinto, D., et al,.
Brazilian Journal of Medical and Biological Research (2008) 41:
99-105)
This
publication list is not all encompassing, and is only meant to
provide limited examples of how Haematologic Technologies' products
are used. We encourage you to search the literature for other
examples pertinent to your experimentation, and to contact us with
any technical questions. |
|
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