|
Description: |
Anti-Human
Osteonectin antibody |
|
Catalog
Number: |
AON-5031 |
|
Type/Host: |
Monoclonal;
mouse. Source: mouse ascites |
|
Subclass: |
IgG1 |
|
Clone
Number: |
AON-5031 |
|
Immunogen: |
Unfractionated
bovine bone extract |
|
Purity: |
>95%
by SDS-PAGE |
|
Epitope: |
First
half of the N-terminus of the molecule excluding the first 28 amino
acids |
|
Dissociation
Constant: |
Not
determined |
|
Inhibitory
Properties: |
Not
determined |
|
Specificity: |
Human,
rat and bovine bone osteonectin, human platelet osteonectin. Cross
reacts with mouse osteonectin analyzed by immunohistochemistry. |
|
Size: |
100
µg; Bulk quantities are available. |
|
Formulation: |
50%
Glycerol/H2O (vol/vol) |
|
Storage: |
-20oC |
|
Shelf
Life (properly stored): |
12
months |
| |
| Applications: |
- Radioimmunoassay
(RIA) - Yes (calcium dependent) |
|
- Blot
- Yes (calcium dependent; suggested starting concentration of 5
µg/mL) |
|
- ELISA
- Yes (calcium dependent) |
|
-
Immunohistochemistry - Yes (calcium dependent) |
|
- Useful
for purification - Yes (calcium dependent) |
| General
Application Guidelines: Western
blot, ELISA,
Immunohistochemistry |
| Each
laboratory should determine an optimum working titer for use in its
particular application. Other applications have not been tested but
use in such assays should not necessarily be excluded. |
| |
|
Customization
options: |
- Reformulation
into the buffer of your choice |
|
- Custom
aliquot sizes |
|
- Conjugation
with FITC, Biotin, HRP and other conjugates |
|
- Coupling
to sepharose and column preparation |
| |
|
Sample Gel:
|
 Gel:
Novex 4-12% Bis-Tris
Load: 1 µg per lane
Buffer:
MOPS
Standard:
SeeBluePlus 2; Myosin (191 kDa), Phosphorylase B (97 kDa), BSA (64
kDa), Glutamic Dehydrogenase (51 kDa), Alcohol Dehydrogenase (39 kDa),
Carbonic Anhydrase (28 kDa), Myoglobin Red (19 kDa), Lysozyme (14
kDa)
|
| |
|
Sample
publications using this antibody:
-
Use in
immunoblot (Koblinski, J., et al.,
Journal of Cell Science 118 (1), 2965-2974 doi:10.1242/jcs.02411)
-
Use
in immunoblot (Mitra, A., et al. Breast Cancer Research
2008, 10:R22 (doi:10.1186/bcr1874))
-
Use
in immunoblot (Robert, G., et al., Cancer Res 2006; 66:
(15). August 1, 2006)
-
Use in
IHC and immunoblot (Tai, I,. et al,.
J. Clin. Invest. 115:1492–1502 (2005). doi:10.1172/JCI23002)
-
Use
in IHC (Pichler, R., et al., AJP Apil 1996 148(4): 1153-1167)
-
Use
in IHC (Eghbali-Fatourechi, G., et al. Bone. 2007 May ; 40(5):
1370–1377)
This
publication list is not all encompassing, and is only meant to
provide limited examples of how Haematologic Technologies' products
are used. We encourage you to search the literature for other
examples pertinent to your experimentation, and to contact us with
any technical questions. |
|
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