|
Description: |
Anti-Human
Prothrombin antibody |
|
Catalog
Number: |
AHP-5013 |
|
Type/Host: |
Monoclonal;
mouse. Source: mouse
ascites. |
|
Subclass: |
IgG2a |
|
Clone
Number: |
AHP-5013 |
|
Immunogen: |
Purified
human prothrombin |
|
Purity: |
>95%
by SDS-PAGE |
|
Epitope: |
Located
in the fragment 2 region (a.a. 156-271) |
|
Dissociation
Constant: |
Not
determined |
|
Inhibitory
Properties: |
Inhibits
clotting and prothrombin activation |
|
Specificity: |
Human
prothrombin, prethrombin-1, fragment 2, meizothrombin. Does not
recognize murine thrombin (Western blot). |
|
Size: |
100
µg; Bulk quantities are available. |
|
Formulation: |
50%
Glycerol/H2O (vol/vol) |
|
Storage: |
-20oC |
|
Shelf
Life (properly stored): |
12
months |
| |
| Applications: |
- Radioimmunoassay
(RIA) - Not determined |
|
- Blot
- Yes (suggested starting concentration of 5 µg/mL) |
|
- ELISA
- Yes |
|
- Useful
for purification - No |
| General
Application Guidelines: Western
blot, ELISA,
Immunohistochemistry |
| Each
laboratory should determine an optimum working titer for use in its
particular application. Other applications have not been tested but
use in such assays should not necessarily be excluded (ex. Immuno
Histochemistry). |
| |
|
Customization
options: |
- Reformulation
into the buffer of your choice |
|
- Custom
aliquot sizes |
|
- Conjugation
with FITC, Biotin, HRP and other conjugates |
|
- Coupling
to sepharose and column preparation |
| |
|
Sample Gel:
|
 Gel:
Novex 4-12% Bis-Tris
Load: 1 µg per lane
Buffer:
MOPS
Standard:
SeeBluePlus 2; Myosin (191 kDa), Phosphorylase B (97 kDa), BSA (64
kDa), Glutamic Dehydrogenase (51 kDa), Alcohol Dehydrogenase (39 kDa),
Carbonic Anhydrase (28 kDa), Myoglobin Red (19 kDa), Lysozyme (14
kDa)
|
| |
|
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PRICING PRODUCT
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