|
Description: |
Anti-Human
Tissue Factor Pathway Inhibitor antibody |
|
Catalog
Number: |
AHTFPI-5138 |
|
Type/Host: |
Monoclonal;
mouse. Source: mouse ascites |
|
Subclass: |
IgG |
|
Clone
Number: |
AHTFPI-5138 |
|
Immunogen: |
Antigen
for this antibody was an N-terminal peptide the first 16 amino acids:
Asp-Ser-Glu-Glu-Asp-Glu-Glu-His-Thr-Ile-Ile-Thr-Asp-Thr-Glu-Cys |
|
Purity: |
>95%
by SDS-PAGE |
|
Epitope: |
The
N-terminal peptide the first 16 amino acids: Asp-Ser-Glu-Glu-Asp-Glu-Glu-His-Thr-Ile-Ile-Thr-Asp-Thr-Glu-Cys |
|
Dissociation
Constant: |
Not
determined |
|
Inhibitory
Properties: |
Not
inhibitory |
|
Specificity: |
Human
Tissue Factor Pathway Inhibitor |
|
Size: |
100
µg; Bulk quantities are available. |
|
Formulation: |
50%
Glycerol/H2O (vol/vol) |
|
Storage: |
-20oC |
|
Shelf
Life (properly stored): |
12
months |
| |
| Applications: |
- Radioimmunoassay
(RIA) - Not determined |
|
- Blot
- Yes (suggested starting concentration of 5 µg/mL) |
|
- ELISA
- Yes |
|
- Useful
for purification - No |
| General
Application Guidelines: Western
blot, ELISA,
Immunohistochemistry |
| Each
laboratory should determine an optimum working titer for use in its
particular application. Other applications have not been tested but
use in such assays should not necessarily be excluded. |
| |
|
Customization
options: |
- Reformulation
into the buffer of your choice |
|
- Custom
aliquot sizes |
|
- Conjugation
with FITC, Biotin, HRP and other conjugates |
|
- Coupling
to sepharose and column preparation |
| |
|
Sample Gel:
|
Gel:
Novex 4-12% Bis-Tris
Load: 1 µg per lane
Buffer:
MOPS
Standard:
SeeBluePlus 2; Myosin (191 kDa), Phosphorylase B (97 kDa), BSA (64
kDa), Glutamic Dehydrogenase (51 kDa), Alcohol Dehydrogenase (39 kDa),
Carbonic Anhydrase (28 kDa), Myoglobin Red (19 kDa), Lysozyme (14
kDa)
|
| |
|
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