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 Sheep Anti-Mouse Prothrombin

PRODUCT AND PURCHASING INFORMATION

 

Description:

Sheep Anti-Mouse Prothrombin

Catalog Number:

PAMFII-S

Type/Host:

Polyclonal; Sheep.  Source: Sheep serum

Description:

Purified IgG fraction

Immunogen:

Purified mouse prothrombin

Purity:

>95% by SDS-PAGE

Inhibitory Properties:

Not determined

Specificity:

Mouse prothrombin, Rat prothrombin, human prothrombin; all confirmed with Western blot/ELISA

Size:

1 and 5 mg; Bulk quantities are available.

Formulation:

50% Glycerol/H2O (vol/vol) 

Storage:

-20oC

Shelf Life (properly stored):

12 months
 
Applications: - Radioimmunoassay (RIA) - Not determined
- Blot - Yes (suggested starting concentration of 10 µg/mL)
- ELISA - Yes (suggested starting concentration of 10 µg/mL)
General Application Guidelines: Western blot, ELISA, Immunohistochemistry
Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded (ex. Immuno Histochemistry).
 

Customization options:

- Reformulation into the buffer of your choice
- Custom aliquot sizes
- Affinity purification
- Conjugation with FITC, Biotin, HRP and other conjugates
- Coupling to sepharose and column preparation
 

Sample Gel:

 

Gel: Novex 4-12% Bis-Tris

Load: 1 µg per lane

Buffer: MOPS

Standard: SeeBluePlus 2; Myosin (191 kDa), Phosphorylase B (97 kDa), BSA (64 kDa), Glutamic Dehydrogenase (51 kDa), Alcohol Dehydrogenase (39 kDa), Carbonic Anhydrase (28 kDa), Myoglobin Red (19 kDa), Lysozyme (14 kDa)

 

 

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