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 ACTIVATION OF FACTOR X BY FACTOR IXa The activation of factor X by the intrinsic factor Xase complex is shown. The
intrinsic factor Xase complex consists of an enzyme, factor IXa, and a cofactor, factor
VIIIa, assembled on a phospholipid surface in the presence of calcium ions. The enzyme
complex proteolytically cleaves a 10,000 molecular weight activation peptide from the NH2-terminal
of the heavy chain of factor X, thus expressing the active-site of factor Xa.
PURCHASING
AND PRODUCT INFORMATION
|
Catalog
Number
HCIXA-0050
HCIXA-EGR
HCIXA-DEGR
BCIXA-1050
BCIXA-EGR
BCIXA-DEGR
MCIXA-5050*
|
Description
Human Factor IXa
Human Factor IXa - EGR
Human Factor IXa - DEGR
Bovine Factor IXa
Bovine Factor IXa - EGR
Bovine Factor
IXa - DEGR
Mouse Factor IXa
|
Size
100
µg
100
µg
100
µg
100
µg
100
µg
100
µg
50
µg |
Formulation
50%
glycerol/water (v/v)
20
mM Hepes, 150 mM NaCl, pH 7.4
20
mM Hepes, 150 mM NaCl, pH 7.4
50%
glycerol/water (v/v)
20
mM Hepes, 150 mM NaCl, pH 7.4
20
mM Hepes, 150 mM NaCl, pH 7.4
50%
glycerol/water (v/v)
|
|
Storage
Glyercol
formulation: -20oC
Hepes
formulation: -80oC |
Purity
>95%
by SDS-PAGE |
Activity
Determination
Factor
IX clotting assay |
Shelf
Life (properly stored)
12
months |
 |
Sample Gel
Information:
Gel:
Novex 4-12% Bis-Tris
Load:
Human Factor IXa, 1 µg per lane
Buffer:
MOPS
Standard:
SeeBluePlus 2; Myosin (191 kDa), Phosphorylase B (97 kDa), BSA (64
kDa), Glutamic Dehydrogenase (51 kDa), Alcohol Dehydrogenase (39 kDa),
Carbonic Anhydrase (28 kDa), Myoglobin Red (19 kDa), Lysozyme (14
kDa)
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|
Sample
publications referencing our Factor IXa:
-
Li,
Y., Nucleic Acids Research, 2006, Vol. 34, No. 22 (Aptimer
interaction)
-
Neuenschwander,
P., et al., J Biol Chem. 2006 August 11; 281(32): 23066–23074.
(Active site titration)
This
publication list is not all encompassing, and is only meant to
provide limited examples of how Haematologic Technologies'
products are used. We encourage you to search the literature
for other examples pertinent to your experimentation, and to
contact us with any technical questions. |
|
U.S.
Pricing |
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inquiry |
SAMPLE
DATA SHEET |
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*Due
to mouse plasma quality issues our mouse proteins are not being
sold until further notice. At the present time we cannot give a
completion date. Thank you.
Overview
of Factor IXa
Factor IXa is produced from its inactive precursor,
factor IX, via proteolytic cleavage by
factor XIa or the tissue factor/factor
VIIa/phospholipid complex. The activation results from the cleavage of two peptide bonds in the factor IX molecule, releasing an activation glycopeptide with an apparent molecular weight of 10,000. The heavy chain of factor IXa (Mr=28,000) contains the serine protease catalytic domain, while the light chain (Mr=17,000) contains the membrane binding domain.
Factor IXa functions as a serine protease involved in the activation of the zymogen,
factor X, to form the enzyme, factor
Xa. The factor IXa enzymatic activity is greatly enhanced by inclusion of its cofactor, factor VIIIa, in the presence of calcium ions on a phospholipid surface. Factor IXa is readily inhibited by
antithrombin III, and this inhibition is greatly accelerated by the presence of heparin. Factor IXa is not inhibited by DFP.
Factor IXa is prepared from highly purified factor IX by activation with factor XIa, as described by Lindquist et al. (5). The factor IXa is further purified by gel filtration, followed by immunoaffinity purification. Factor IXa is also available with the active site irreversibly blocked by the tripeptide chloromethyl ketone,
EGRck, or by the fluorescent inhibitor, Dansyl-EGRck. It is supplied in 50% (vol/vol)
glycerol/H2O for storage at -20°C. Purity is assessed by SDS-PAGE analysis. Activity is determined in a one-stage clotting assay.
Properties of
Factor IXa
| Localization: |
Plasma |
| Mode of action: |
Enzyme component of the Factor Xase complex |
| Molecular weight: |
45,000 (7) |
| Extinction coefficient: |
|
| Structure: |
2 subunits,
Mr=28,000 and 17,000 (5), NH2-terminal gla-domain, two EGF
domains |
| Post-translational modifications |
one b-hydroxyaspartate (3), twelve gla residues (4) |
PURCHASING
AND PRODUCT INFORMATION
|
Catalog
Number
HCIXA-0050
HCIXA-EGR
HCIXA-DEGR
BCIXA-1050
BCIXA-EGR
BCIXA-DEGR
MCIXA-5050
|
Description
Human Factor IXa
Human Factor IXa - EGR
Human Factor IXa - DEGR
Bovine Factor IXa
Bovine Factor IXa - EGR
Bovine Factor
IXa - DEGR
Mouse Factor IXa
|
Size
100
µg
100
µg
100
µg
100
µg
100
µg
100
µg
50
µg |
Formulation
50%
glycerol/water (v/v)
20
mM Hepes, 150 mM NaCl, pH 7.4
20
mM Hepes, 150 mM NaCl, pH 7.4
50%
glycerol/water (v/v)
20
mM Hepes, 150 mM NaCl, pH 7.4
20
mM Hepes, 150 mM NaCl, pH 7.4
50%
glycerol/water (v/v)
|
|
Storage
Glyercol
formulation: -20oC
Hepes
formulation: -80oC |
Purity
>95%
by SDS-PAGE |
Activity
Determination
Factor
IX clotting assay |
Shelf
Life (properly stored)
12
months |
|
U.S.
Pricing |
Product
inquiry |
SAMPLE
DATA SHEET |
ORDER
NOW! |
References
1. Thompson, A.R., Blood, 67, 565 (1986).
2. Hedner, U., et al., In Hemostasis and Thrombosis, 2nd edition, ed. R.W. Colman, J. Hirsh, V.J. Marder, E.W. 3. Salzman, pp., 39-47. J.P. Lippincott Co, Philadelphia, 1987.
4. Discipio, R.G., et al., Biochemistry, 16, 698 (1977).
5. McMullen, B.A., et al., BBRC, 115, 8 (1983).
6. Lindquist, P.A., et al., J. Biol. Chem., 253, 1902 (1978).
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