|
Snake
venom proteases are useful tools for studying coagulation reactions.
The venom proteases offered are highly purified, homogenous preparations
with the indicated activities. All of our venom products are
supplied in 50% glycerol for storage at -20oC.
|
RVV-V ACTIVATOR |
RVV-X ACTIVATOR |
ECV-
PROTHROMBIN
ACTIVATOR |
|
CATALOG #
|
RVVV-2000 |
RVVX-2010 |
ECVII-2011 |
|
SIZE
|
100
µg |
100
µg |
100
µg
|
|
BUFFER
|
50%
glycerol/water (v/v)
|
50%
glycerol/water (v/v)
|
50%
glycerol/water (v/v)
|
|
STORAGE
|
-20oC |
-20oC |
-20oC
|
|
SOURCE
|
Vipera Russelli
Russelli |
Vipera Russelli
Russelli |
Vipera Echis
Carinatus
|
|
MOLECULAR WEIGHT
|
Mr = 27,200 single
chain glycoprotein |
Mr = 79,000
glycoprotein 2 disulfide linked subunits
(Mr = 67kD, 26kD) |
Mr = 56,000
single
chain
|
|
CLASS |
serine protease |
Zn+2
dependant endopeptidase |
metalendo-
peptidase |
|
INHIBITORS |
DFP |
EDTA, o-phenanthroline |
EDTA, DTT
o-phenanthroline |
|
APPLICATION |
converts single
chain factor V to an active two chain form |
Conversion of
factor X to Xa, factor
IX to IXaα |
Cleavage of
Arg332-Ile323
bond in
prothrombin
to
form
meizothrombin |
|
USE |
start
at a 1:500 (enzyme:substrate) mass ratio for
activation (e.g. 2 ug enzyme per 1 mg of
material activated). Exact ratios and
conditions must be determined in your lab. |
|
RVV-V
|
RVV-X
|
Sample Gel
Information:
Gel:
Novex 4-12% Bis-Tris
Load:
RVV-V and RVV-X, 1 µg per lane
Buffer:
MOPS
Standard:
SeeBluePlus 2; Myosin (191 kDa), Phosphorylase B (97 kDa), BSA (64
kDa), Glutamic Dehydrogenase (51 kDa), Alcohol Dehydrogenase (39 kDa),
Carbonic Anhydrase (28 kDa), Myoglobin Red (19 kDa), Lysozyme (14
kDa)
|
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Sample
publications referencing our Venom Proteases:
-
Dasgupta,
S., et al., Blood. 2009 113:
1332-1339. (RVVX in thrombin generation)
This
publication list is not all encompassing, and is only meant to
provide limited examples of how Haematologic Technologies'
products are used. We encourage you to search the literature
for other examples pertinent to your experimentation, and to
contact us with any technical questions. |
|
U.S.
Pricing |
Product
inquiry |
SAMPLE
DATA SHEETS |
ORDER
NOW! |
References
-
Kisiel, W., et.al., Biochen., 15, 4901-4905 (1976).
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Kisiel, W. and Canfield, R., Meth.Enzymol., 80, 275-285
(1980).
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Kane, W. and Majerus, P.J., J.Biol.Chem., 256, 1002-1007
(1987).
-
Morita, T. and Iwanaga, S., Meth.Enzymol., 80, 303-311
(1980).
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