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HUMAN PLASMINOGEN The domain structure of human plasminogen is represented where: K1-K5
= the 5 kringle domains, B-CHAIN = catalytic domain of plasmin , and the arrows
indicate the sites of proteolytic cleavage by plasmin,
elastase, and plasminogen
activators (PA'S).
PURCHASING
AND PRODUCT INFORMATION
|
Catalog
Number
HCPG-0130 HCPG-0131 HCPG-0132 HCPG-0133 BCPG-1130 MCPG-5130*
|
Description
Human
glu-Plasminogen Human
glu-Plasminogen CHOI Human glu-Plasminogen CHOII Human
lys-Plasminogen Bovine
glu-Plasminogen Mouse
glu-Plasminogen |
Size
1
milligram
1
milligram
1
milligram
1
milligram
1
milligram
100
µg |
Formulation
50% (vol/vol) glycerol/H2O
50%
(vol/vol) glycerol/H2O
50%
(vol/vol) glycerol/H2O
50%
(vol/vol) glycerol/H2O
50%
(vol/vol) glycerol/H2O
50%
(vol/vol) glycerol/H2O |
|
Storage
-20oC |
Purity
>95%
by SDS-PAGE |
Activity
Determination
<
10 ppm plasmin activity for glu-PG |
Shelf
Life (properly stored)
12
months |
|
Sample
SDS-PAGE
Lane
1: Human Glu-Plasminogen (HCPG-0130) Reduced
Lane
2: Human Glu-Plasminogen CHOI (HCPG-0131) Reduced
Lane
3: Human Glu-Plasminogen CHOII (HCPG-0132) Reduced
Lane
4: Human Lys-Plasminogen (HCPG-0133) Reduced
Marker:
See Blue +2_MOPS
Lane
5: Human Glu-Plasminogen (HCPG-0130) Non-Reduced
Lane
6: HumanGlu-Plasminogen CHOI (HCPG-0131) Non-Red
Lane
7: Human Glu-Plasminogen CHOII (HCPG-0132) Non-Red
Lane
8: Human Lys-Plasminogen (HCPG-0133) Non-Reduced
Gel:
Novex 4-12% Bis-Tris
Load:
1 µg per lane
Buffer:
MOPS
Standard:
SeeBluePlus 2; Myosin (191 kDa), Phosphorylase B (97 kDa), BSA (64
kDa), Glutamic Dehydrogenase (51 kDa), Alcohol Dehydrogenase (39 kDa),
Carbonic Anhydrase (28 kDa), Myoglobin Red (19 kDa), Lysozyme (14
kDa)
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Sample
publications referencing our Plasminogen:
-
Zaas
AK, Liao G, Chien JW, Weinberg C, Shore D, et al. (2008)
Plasminogen Alleles Influence Susceptibility to Invasive
Aspergillosis. PLoS Genet 4(6):
e1000101.doi:10.1371/journal.pgen.1000101 (mouse plasminogen)
This
publication list is not all encompassing, and is only meant to
provide limited examples of how Haematologic Technologies' products
are used. We encourage you to search the literature for other
examples pertinent to your experimentation, and to contact us with
any technical questions. |
|
U.S.
Pricing |
Product
inquiry |
SAMPLE
DATA SHEET |
ORDER
NOW! |
*Due
to mouse plasma quality issues
our mouse proteins are not being sold until further
notice. At the
present time we cannot give a completion date. Thank you.
Overview
of Plasminogen
Plasminogen is a single chain glycoprotein zymogen which is synthesized in the liver and circulates in plasma at a concentration of approximately 2.4
µM (1,2). The plasminogen molecule contains 790 amino acids, 24 disulfide bridges, no free sulfhydryls and 5 regions of internal sequence homology, known as kringles, between Lys77 and Arg560. These five triple-looped, three disulfide bridged, kringle regions are homologous to the kringle domains in t-PA, u-PA and
prothrombin. Plasminogen contains one high affinity (Kd=9x10-6M) and four low affinity (Kd=5x10-3M) lysine binding sites. The high affinity binding site resides within the first kringle region of plasminogen. The interaction of plasminogen with fibrin and
α2-antiplasmin is mediated by these lysine binding sites. Native glu-plasminogen (Mr=88,000) is readily converted to Lys-77-plasminogen (Mr=83,000) by
plasmin hydrolysis of the Lys76-Lys77 peptide bond. Elastase catalyzed cleavage of the Val441-Val442 peptide bond of glu-plasminogen yields a functionally active zymogen termed Val-442 plasminogen or mini-plasminogen.
The conversion of plasminogen to plasmin occurs by a variety of mechanisms, but all result in hydrolysis of the Arg560-Val561 peptide bond of plasminogen, yielding two chains which remain covalently associated by a disulfide bond.
Native glu-plasminogen is prepared from fresh frozen human plasma by a modification of the procedure of Castellino (3), utilizing gel filtration and affinity chromatography. The two carbohydrate variants of glu-plasminogen (CHOI and CHOII) are isolated by gradient elution from lysine-Sepharose using the lysine analog, e-aminocaproic acid (3). The plasminogen is supplied in 50% (vol/vol) glycerol/H2O for storage at
-20oC. Purity is determined by SDS-PAGE analysis.
Properties of
Plasminogen
| Localization: |
Plasma |
| Plasma concentration: |
2.4 µM (human) (4) |
| Mode of action: |
Zymogen; precursor to the serine protease plasmin |
| Molecular weight: |
88,000 (glu
plasminogen) (5)
83,000 (lys-plasminogen) (5)
38,000 (val-plasminogen) (6) |
| Extinction coefficient: |
|
| Isoelectric point: |
6.2 (glu-plasminogen) (1)
6.7-8.3 (lys-plasminogen) (1) |
| Structure: |
single chain, 24 intra chain disulfide bridges, 5 kringle regions. |
| Percent carbohydrate: |
Approximately 2% |
PURCHASING
AND PRODUCT INFORMATION
|
Catalog
Number
HCPG-0130 HCPG-0131 HCPG-0132 HCPG-0133 BCPG-1130 MCPG-5130
|
Description
Human
glu-Plasminogen Human
glu-Plasminogen CHOI Human glu-Plasminogen CHOII Human
lys-Plasminogen Bovine
glu-Plasminogen Mouse
glu-Plasminogen |
Size
1
milligram
1
milligram
1
milligram
1
milligram
1
milligram
100
µg |
Formulation
50% (vol/vol) glycerol/H2O |
|
Storage
-20oC |
Purity
>95%
by SDS-PAGE |
Activity
Determination
<
10 ppm plasmin activity for glu-PG |
Shelf
Life (properly stored)
12
months |
|
U.S.
Pricing |
Product
inquiry |
SAMPLE
DATA SHEET |
ORDER
NOW! |
References
1. Robbins, K.C., Methods in Enzymology, 45, 257 (1976).
2. Collen, D. in Blood Coagulation, Zwaal, R.E.A. and Hemker, H.C., eds., pp. 243-258, New York, Elsevier (1986).
3. Castellino, F.J., et al., Methods in Enzymology, 80, 365 (1981).
4. Wohl, R.C., et al., Thromb. Res., 27 523 (1982).
5. Barlow, G.H., et al., Biochemistry, 23, 2384 (1984).
6. Sottrup-Jensen, L., et al., in Progress in Chemical Fibrinolysis and Thrombolysis, Vol. 3, ed. J.F. Davidson, 7. R.M. Rowan, M.M. Samana, P.C. Desnoyers, pp. 197-228, New York: Raven Press (1975).
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