Snake Venom Proteases

• RVVV-2000 RVV-V Activator Price $47.00/100 µg ($43.00/min. 5)

  Size	100 µg
  Formulation	50% glycerol/water (v/v)

  Storage	-20°C
  Shelf Life (properly stored)	12 months

  Molecular Weight	27,200 - single chain glycoprotein
  Source	Vipera Russelli Russelli

  Class	serine protease
  Inhibitors	DFP

  Application	converts single chain factor V to an active two chain form
  Use	start at a 1:500 (enzyme:substrate) mass ratio for activation  (e.g. 2 ug enzyme per 1 mg of material activated).  Exact ratios and conditions must be determined in your lab.
  Notes	NOT tissue/cell culture grade. NOT tested for endotoxin.

• RVVX-2010 RVV-X Activator Price $47.00/100 µg ($43.00/min. 5)

  Size	100 µg
  Formulation	50% glycerol/water (v/v)

  Storage	-20°C
  Shelf Life (properly stored)	12 months

  Molecular Weight	79,000 glycoprotein 2 disulfide linked subunits (Mr = 67kD, 26kD)
  Source	Vipera Russelli Russelli

  Class	Zn+2 dependant endopeptidase
  Inhibitors	EDTA, o-phenanthroline

  Application	Conversion of factor X to Xa, factor IX to IXaα
  Use	start at a 1:500 (enzyme:substrate) mass ratio for activation  (e.g. 2 ug enzyme per 1 mg of material activated).  Exact ratios and conditions must be determined in your lab.
  Notes	NOT tissue/cell culture grade. NOT tested for endotoxin.

• ECVVII-2011 Echis Carinatus Prothrombin Activator (Echarin) Price $151.00/100 µg ($137.00/min. 5)

  Size	100 µg
  Formulation	50% glycerol/water (v/v)

  Storage	-20°C
  Shelf Life (properly stored)	12 months

  Molecular Weight	56,000, single chain
  Source	Vipera Echis Carinatus

  Class	metalendo-peptidase
  Inhibitors	EDTA, DTT, o-phenanthroline

  Application	Cleavage of Arg323-Ile324 bond in prothrombin to form meizothrombin
  Use	start at a 1:500 (enzyme:substrate) mass ratio for activation  (e.g. 2 ug enzyme per 1 mg of material activated).  Exact ratios and conditions must be determined in your lab.
  Notes	NOT tissue/cell culture grade. NOT tested for endotoxin.

• Sample Gel Information
• Overview
• Properties
• References
• Sample Publications

Gel	Novex 4-12% Bis-Tris
Load	RVV-V and RVV-X, 1 µg per lane
Buffer	MOPS
Standard	SeeBluePlus 2; Myosin (191 kDa), Phosphorylase B (97 kDa), BSA (64 kDa), Glutamic Dehydrogenase (51 kDa), Alcohol Dehydrogenase (39 kDa), Carbonic Anhydrase (28 kDa), Myoglobin Red (19 kDa), Lysozyme (14 kDa)

No overview available.

No properties available.

1. Kisiel, W., et.al., Biochen., 15, 4901-4905 (1976).
2. Kisiel, W. and Canfield, R., Meth.Enzymol., 80, 275-285 (1980).
3. Kane, W. and Majerus, P.J., J.Biol.Chem., 256, 1002-1007 (1987).
4. Morita, T. and Iwanaga, S., Meth.Enzymol., 80, 303-311 (1980).

1. Dasgupta, S., et al., Blood. 2009 113: 1332-1339. (RVVX in thrombin generation)

This publication list is not all encompassing, and is only meant to provide limited examples of how Haematologic Technologies' products are used. We encourage you to search the literature for other examples pertinent to your experimentation, and to contact us with any technical questions.