Snake Venom Proteases
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Price $51.00/100 µg ($46.00/min. 5)
Size 100 µg Formulation 50% glycerol/water (v/v) Storage -20°C Shelf Life (properly stored) 12 months Molecular Weight 27,200 - single chain glycoprotein Source Vipera Russelli Russelli Class serine protease Inhibitors DFP Application converts single chain factor V to an active two chain form Use start at a 1:500 (enzyme:substrate) mass ratio for activation (e.g. 2 ug enzyme per 1 mg of material activated). Exact ratios and conditions must be determined in your lab. Notes NOT tissue/cell culture grade. NOT tested for endotoxin. -
Price $51.00/100 µg ($46.00/min. 5)
Size 100 µg Formulation 50% glycerol/water (v/v) Storage -20°C Shelf Life (properly stored) 12 months Molecular Weight 79,000 glycoprotein 2 disulfide linked subunits (Mr = 67kD, 26kD) Source Vipera Russelli Russelli Class Zn+2 dependant endopeptidase Inhibitors EDTA, o-phenanthroline Application Conversion of factor X to Xa, factor IX to IXaα Use start at a 1:500 (enzyme:substrate) mass ratio for activation (e.g. 2 ug enzyme per 1 mg of material activated). Exact ratios and conditions must be determined in your lab. Notes NOT tissue/cell culture grade. NOT tested for endotoxin. -
Price $163.00/100 µg ($148.00/min. 5)
Size 100 µg Formulation 50% glycerol/water (v/v) Storage -20°C Shelf Life (properly stored) 12 months Molecular Weight 56,000, single chain Source Vipera Echis Carinatus Class metalendo-peptidase Inhibitors EDTA, DTT, o-phenanthroline Application Cleavage of Arg323-Ile324 bond in prothrombin to form meizothrombin Use start at a 1:500 (enzyme:substrate) mass ratio for activation (e.g. 2 ug enzyme per 1 mg of material activated). Exact ratios and conditions must be determined in your lab. Notes NOT tissue/cell culture grade. NOT tested for endotoxin.
No overview available.
| Gel | Novex 4-12% Bis-Tris |
|---|---|
| Load | RVV-V and RVV-X, 1 µg per lane |
| Buffer | MOPS |
| Standard | SeeBluePlus 2; Myosin (191 kDa), Phosphorylase B (97 kDa), BSA (64 kDa), Glutamic Dehydrogenase (51 kDa), Alcohol Dehydrogenase (39 kDa), Carbonic Anhydrase (28 kDa), Myoglobin Red (19 kDa), Lysozyme (14 kDa) |
No properties available.
- Kisiel, W., et.al., Biochen., 15, 4901-4905 (1976).
- Kisiel, W. and Canfield, R., Meth.Enzymol., 80, 275-285 (1980).
- Kane, W. and Majerus, P.J., J.Biol.Chem., 256, 1002-1007 (1987).
- Morita, T. and Iwanaga, S., Meth.Enzymol., 80, 303-311 (1980).
- Dasgupta, S., et al., Blood. 2009 113: 1332-1339. (RVVX in thrombin generation)
This publication list is not all encompassing, and is only meant to provide limited examples of how Haematologic Technologies' products are used. We encourage you to search the literature for other examples pertinent to your experimentation, and to contact us with any technical questions.